%20(1080%20x%20360%20px).png)
Cronobacter & Salmonella
Home / Products / Microbiology Culture Media / Cranobacter & Salmonella
Biokar CSD Method – Cronobacter Detection
The Biokar CSD Method is a validated rapid detection system for Cronobacter spp. in powdered infant formula, raw materials, and production environment samples. It combines a selective enrichment phase using CSD supplement with subsequent plating on CCI Agar, producing clear blue to blue-green colonies for presumptive Cronobacter detection within 37–39 hours. This method is NF VALIDATION certified according to ISO 16140-2 and fully complies with ISO 22964.
Importantly, the same enrichment broth used in the CSD method can also support simultaneous Salmonella detection via the Biokar IRIS Salmonella® method—making it an efficient dual-target workflow for critical microbiological monitoring in infant nutrition industries.
Salmonella Enrichment Buffer
> Designed for Acidic Products:
-
Ideal for food and feed with pH lower than 4.5.
> Double-Strength Buffered Peptone Water:
-
Ensures effective pre-enrichment of Salmonella.
> Standards Compliant:
-
NF EN ISO 6579-1 (Detection and serotyping of Salmonella spp.)
-
NF EN ISO 6887-1 & 6887-4 (Sample prep and dilution protocols)
> Validated for Rapid Methods:
-
Compatible with IRIS Salmonella® and SESAME Salmonella TEST®.
> Versatile Use:
-
Can be used in any method requiring double-strength Buffered Peptone Water.
.png)
This double-strength buffered enrichment medium is specially developed to enhance the detection and recovery of Salmonella in acidic food and feed products, providing optimal growth conditions even in low pH environments.
CSD Method
CSD Method for Cronobacter Detection:
> Quick Detection:
-
Detects Cronobacter in milk powders (with or without probiotics), infant ingredients, and environmental samples.
> Easy Confirmation:
-
After enrichment, use CCI agar to confirm the presence of Cronobacter.
> Certified Method:
-
NF VALIDATION certified for detecting Cronobacter in milk powders, ingredients, and environmental samples.
Applicable for:
> Infant milk powders (with or without probiotics) and ingredients in amounts of 0 to 50 g and 50 to 375 g.
> Production environment samples.
> ISO Compliant:
-
Follows ISO 22964: 2017 for accurate Cronobacter detection.
> Enriches Both Salmonella & Cronobacter:
-
CSD supplement helps enrich both Salmonella and Cronobacter in milk powders and environmental samples.
> Standard Methods:
-
Based on EN ISO 22964: 2017 for Cronobacter and the IRIS method for Salmonella.
The CSD method provides a rapid and efficient way to detect Cronobacter spp. in human food products as well as in samples from the production environment.
Chromogenic Cronobacter Isolation Agar (CCI)
.png)
> Detection of Cronobacter spp.:
-
Used to identify Cronobacter species, including Cronobacter sakazakii, in food products and ingredients for human consumption and animal feed.
> Environmental Control:
-
Suitable for controlling production environment samples to ensure safety.
> Specialized for Infant Foods:
-
Specifically effective for detecting Cronobacter in milk powder, dehydrated products, and components in infant foods.
> ISO Compliant:
-
The formulation of Chromogenic Cronobacter Isolation Agar complies with the ISO 22964 directive for accurate detection.
CCI Agar is designed for the detection of microorganisms in food products and ingredients that are intended for both human consumption and animal feed, ensuring safety and quality.